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生物力學(xué)相關(guān)產(chǎn)品

單層細(xì)胞壓縮力加載培養(yǎng)與實(shí)時(shí)觀察分析系統(tǒng)

型號(hào):cell confiner
聯(lián)系人:李先生
聯(lián)系電話:18618101725
品牌:法國(guó)cell confinement

單層細(xì)胞壓縮力加載培養(yǎng)與實(shí)時(shí)觀察分析系統(tǒng)-

細(xì)胞被均勻地限制/壓縮在兩個(gè)亞微米分辨率的兩個(gè)平行表面之間。
不同的限制高度（例如1um – 300um）
允許長(zhǎng)期細(xì)胞培養(yǎng)和細(xì)胞增殖，同時(shí)保持對(duì)封閉的完美控制
與高分辨率光學(xué)顯微鏡系統(tǒng)兼容
可以處理足夠多的細(xì)胞以進(jìn)行完整的基因表達(dá)分析
可與生物功能化的微結(jié)構(gòu)化底物和/或不同的基質(zhì)（幾何形狀控制）結(jié)合使用
可以與凝膠結(jié)合（硬度控制）
兼容任何細(xì)胞培養(yǎng)底物（培養(yǎng)皿至96孔板）

產(chǎn)品：

應(yīng)用：

Cell migration 2.5D, migration and interaction of non-adhesive cells, cell squeezing, imaging of flat cells (organelles aligned in 2D), super-resolution video-microscopy (organelles move less), contractility assay, etc
Confinement illustration
HeLa cells: not confined, 5 ?m, 3 ?m.
Explore examples of applications

> Cancer invasiveness assay: Quantification of migration behaviors and migration transitions
> Cancer aggressiveness assay: Quantification of contractility of somatic or cancer cells
> Endocytosis assay: Improved observation of events taking place at the membrane
> Exocytosis assay: Improved observation of events taking place at the apical membrane
> Frustrated phagocytosis: Characterization of the mechanism
> Immune system in a well: 2D migration and interaction of non-adherent immune cells
> Immune cells interaction: 2D interaction of non-adherent immune cells
> Mitotic assembly assay: Quantification of mitotic spindle disorders
> Quantitative cell migration assay: Fast and fine analysis of cell migration properties

文獻(xiàn)：

PUBLICATIONS

Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672
Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386
Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101
Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283
Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414
Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169
Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29

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